Christopher McCulloch

Director, Matrix Dynamics Group, Faculty of Dentistry, University of Toronto DEGREES & AWARDSPUBLICATIONS

Degrees & Awards

  • DDS 1976; Cert. Periodontics 1978; PhD 1982; FRCD(C.) 1982

Current Position

  • Professor, Matrix Dynamics Group, Faculty of Dentistry, University of Toronto

Honours, Distinctions and Awards

  • 04/2009 International Association of Dental Research (IADR) Distinguished Scientist Award for Research in Oral Biology, United States (supported by Church & Dwight Company)
  • 06/2009 International Association of Dental Research (IADR) Hatton Award Supervisor (Graduate Student, Hugh Kim)
  • 09/2007 – present Fellow, Canadian Academy of Health Sciences
  • 10/2006 – 09/2013 Canada Research Chair, Tier 1 Research Award, CRC Program, Canada
  • 08/1998 – 09/1998 Honorary Award for Excellence in Research, Columbia University,
    United States
  • 04/1998 International Association of Dental Research (IADR) Hatton Award Supervisor (Graduate Student, Michael Glogauer)
  • 07/1989 – 06/1994 Renewal of Career Scientist Award, Ontario Ministry of Health, Canada
  • 07/1984 – 06/1989 Career Scientist Award, Ontario Ministry of Health, Canada
  • 1982 – 1984 MRC Fellowship; Supervisor- H. Cheng
  • 1980 – 1982 MRC Fellowship; Supervisor- A.H. Melcher
  • 01/1978 – 12/1982 First Prize, Research in Cell Migration Research Award, Canadian Dental Association, Canada

Areas Of Expertise

Remodelling of connective tissue matrices. Chris initially established the location and proliferative behaviour of cells in the periodontal ligament and showed that subpopulations of these cells can undergo self-renewal, a characteristic of stem cells. This work is widely quoted in the connective tissue cell literature. He used actin isoform analyses to establish that a-smooth muscle actin is a differentiation marker for contractile fibroblasts from the periodontium and is required for collagen contraction. Further, he has shown that the actin binding protein filamin A is required for protection of cells in mechanically active environments. Finally, Chris has established an in vitro model for collagen phagocytosis and has shown the mechanisms by which phagocytosis is important in the formation of fibrotic lesions.

 

Current Grants

  • 10/2015 – 09/2020 Canadian Institutes of Health Research (CIHR)
    Transitional Operating Grant
    Title: Characterizing the role of Adseverin in Osteoclastogenesis
    Principle Applicant: M. Glogauer; co-applicant: C. McCulloch.
    Award: $863,500; Duration of Award: 5 years.
  • 10/2014 – 09/2019 Canadian Institutes of Health Research (CIHR)
    Operating Grant
    Title: Role of Flightless in Matrix Remodeling and Gingival Overgrowth
    Principle Applicant: Christopher McCulloch; Total award: $677,105
    Rank in Committee: 4/56; Rating 4.64
  • 11/2013 – 10/2020 Canadian Institutes of Health Research (CIHR)
    Canada Research Chairs (CRC)
    Title: Canada Research Chair in Matrix Dynamics – Research Chair
    Principal Applicant: McCulloch, CAG; Total award: $1,400,000.
  • 10/2012 – 09/2017 Canadian Institutes of Health Research (CIHR)
    Operating Grant
    Title: Preservation of Gingival Health and Adhesion Signaling
    Principal Applicant: McCulloch, CAG; Total Award: $678,125

Hot Protocols

Electroinjection of antibodies to block cytoskeletal protein function in cell migration

To optimize the method by which antibodies can inhibit the intracellular activity of actin severing proteins such as gelsolin, in vitro studies were performed first to determine the amount of antibody that would complex known amounts of gelsolin. From estimates of cell number and the amount of protein in the lysates we determined the optimal concentration of purified gelsolin for experiments (1 µM; i.e. 83 µg/ml). Gelsolin was incubated with various dilutions of gelsolin antibody (mouse monoclonal anti-gelsolin antibody; 2C4; from Sigma in electroporation buffer). The gelsolin-antibody complexes were removed with protein A sepharose. The residual, free gelsolin was detected by immunoblotting and the optimal concentration of antibody for complete complexing was calculated (100 µg/ml). For electroporation and severing assays an equivalent molar amount of antibody was used.

The requirement for gelsolin in cell migration in the chemotactic assay

To assess directly the requirement for gelsolin in cell migration in the chemotactic assay, the gelsolin antibody or an irrelevant antibody (pan-cytokeratin antibody, clone PCK-26; diluted in electroporation buffer) were electroinjected into cells by electroporation with a Bio-Rad gene pulser, a capacitance extender and sterile cuvettes. An 800-ul aliquot of 2 X 106 cells/ml was placed into an electroporation cuvette (0.4 cm inter-electrode distance) and the cells were electroporated in a buffer containing 5.37 mM KCl, 0.52 mM KH2PO4, 0.64 mM MgCl2, 0.63 mM MgSO4, 85.5 mM NaCl, 5.8 mM NaHCO3, 0.50 mM NaH2PO4, and 12.5 mM HEPES at a field strength of 250 V/cm and capacitance of 500 µF. The cells were collected by centrifugation, suspended in growth medium without serum and 5X104 cells/well were assayed in the Boyden chamber with a chemotactic gradient for various time periods. The filter-attached cells were fixed and stained with DAPI before counting. About 108 antibody molecules per cell can be introduced into viable cells using this method and that electroinjected antibodies can specifically bind and inactivate specific functional proteins.

Measurement of calcium ion concentration in specific cellular compartments

For analysis of calcium in specific cellular compartments, cells were incubated with 4.5 mM mag-fura 2/AM for 140 mins at 37°C to load ER stores. Estimates of ER store calcium were made with a microscope-based, ratio fluorimeter (Photon Technology Inc., London, ON) in single cells. For mitochondrial calcium, Rat2 fibroblasts were loaded with 4.5 mM rhod-2/AM in 0.005% (v/v) Pluronic gel for 30-140 mins at 37°C. The cells were washed twice and incubated in calcium buffer for imaging. The magnitude of fluorescence of Rhod 2-stained samples was observed in an inverted microscope equipped with a CCD camera (Pentamax; Princeton, NJ) and analysed using the Winview software program (Princeton). As ratio imaging cannot be used to overcome problems of dye leakage and photobleaching in time course experiments with rhod-2, single excitation/single emission imaging analyses were conducted with a number of corrective procedures. First, the fluorescence intensity of rhod-2 was measured in small (~4 mm2) sampling grids in the lamellopodia of well-spread cells to avoid the inclusion of nucleoli which stain brightly with rhod 2. Second, background fluorescence and adjustments for time-dependent photo-bleaching were made for each measurement by calculating the bleach-induced rate constant (i.e. time-dependent loss of fluorescence) in untreated cell samples that were not incubated with collagen-coated beads. Third, for verification of the appropriate spatial localization of the rhod-2 staining and to ensure that rhod-2 was not sequestered to lysosomes, in some experiments Rat2 fibroblasts were co-loaded with 100 nM MitoTracker Green and 4.5 mM rhod-2/AM in 0.005% (v/v) Pluronic for 30 minutes. The cells were washed twice and left in calcium buffer before imaging. These loading protocols facilitate measurements of store-specific calcium after stimulation by a variety of ligands including collagen or fibronectin ligands.

Publications

Click corresponding year below for publications.

2017

Cao Y, Wang Y, Sprangers S, Picavet DI, Glogauer M, McCulloch CA, Everts V. Deletion of Adseverin in Osteoclasts Affects Cell Structure But Not Bone Metabolism.  Calcif Tissue Int. 2017 Apr 7. Epub ahead of print.

Coelho NM, Arora PD, van Putten S, Boo S, Petrovic P, Lin AX, Hinz B, McCulloch CA. Discoidin Domain Receptor 1 Mediates Myosin-Dependent Collagen Contraction. Cell Rep. 2017 Feb 14;18(7):1774-1790.

Civitarese RA, Kapus A, McCulloch CA, Connelly KA. Role of integrins in mediating cardiac fibroblast-cardiomyocyte cross talk: a dynamic relationship in cardiac biology and pathophysiology. Basic Res Cardiol. 2017 Jan;112(1):6.

 

2016

Embry AE, Mohammadi H, Niu X, Liu L, Moe B, Miller-Little WA, Lu CY, Bruggeman LA, McCulloch CA, Janmey PA, Miller RT. Biochemical and Cellular Determinants of Renal Glomerular Elasticity. PLoS One. 2016 Dec 12;11(12):e0167924.

Rajshankar D, Wang Y, McCulloch CA. Osteogenesis requires FAK-dependent collagen synthesis by fibroblasts and osteoblasts. FASEB J. 2017 Mar;31(3):937-953.

Mezawa M, Pinto VI, Kazembe MP, Lee WS, McCulloch CA. Filamin A regulates the organization and remodeling of the pericellular collagen matrix. FASEB J. 2016 Oct;30(10):3613-3627.

Coelho NM, McCulloch CA. Contribution of collagen adhesion receptors to tissue fibrosis. Cell Tissue Res. 2016 Sep;365(3):521-38. Review.

Civitarese RA, Talior-Volodarsky I, Desjardins JF, Kabir G, Switzer J, Mitchell M, Kapus A, McCulloch CA, Gullberg D, Connelly KA.  The a11 integrin mediates fibroblast- extracellular matrix- cardiomyocyte interactions in health and disease. Am J Physiol Heart Circ Physiol. 2016 Jul 1;311(1):H96-H106.

D.C. Matthews, K. McNeil, M. Brillant, C. Tax, P. Maillet, C.A. McCulloch, and M. Glogauer. Factors Influencing Adoption of New Technologies into Dental Practice: A Qualitative Study. Original Report: Qualitative Research, Vol. 1, Issue 1. International & American Associations for Dental Research 2016. April 2016.

Wang Q, Wang Y, Downey GP, Plotnikov S, McCulloch CA. A ternary complex comprising FAK, PTPα and IP3 receptor 1 functionally engages focal adhesions and the endoplasmic reticulum to mediate IL-1-induced Ca2+ signalling in fibroblasts. Biochem J. 2016 Feb 15;473(4):397-410.

 

2015

Christopher A. McCulloch. Historical Perspective of Periodontal Progenitor Cells: Early Studies That Clarified Identity and Function. Curr Oral Health Rep (2015) 2:227–235.

Mohammadi H, Pinto VI, Wang Y, Hinz B, Janmey PA, McCulloch CA. Filamin A Mediates Wound Closure by Promoting Elastic Deformation and Maintenance of Tension in the Collagen Matrix. J Invest Dermatol. 2015 Nov;135(11):2852-61.

Pinto VI, Mohammadi H, Lee WS, Cheung A, McCulloch CA. PAK1 is involved in sensing the orientation of collagen stiffness gradients in mouse fibroblasts. Biochim Biophys Acta. 2015 Oct;1853(10 Pt A):2526-38.

Arora PD, Wang Y, Bresnick A, Janmey PA, McCulloch CA. Flightless I interacts with NMMIIA to promote cell extension formation that enables collagen remodeling. Mol Biol Cell. 2015 Jun 15;26(12):2279-97.

Wang Y, Brooks PJ, Jang JJ, Silver AS, Arora PD, McCulloch CA, Glogauer M. Role of actin filaments in fusopod formation and osteoclastogenesis. Biochim Biophys Acta. 2015 Jul;1853(7):1715-24.

L.A. Staudinger, S.J. Spano, W.S. Lee, N. Coelho, T.J. Moriarty, C.A. McCulloch. Role of discoidin domain receptor 1 in dysregulation of collagen remodeling by cyclosporin A. Int J Biochem Cell Biol. 2015 May;62:80-7.

Cheung JW, Jain D, McCulloch C, Santerre P. Pro-angiogenic Character of Endothelial Cells and Gingival Fibroblasts Co-cultures in Perfused Degradable Polyurethane (D-PHI) Scaffolds. Tissue Eng Part A. 2015 May;21(9-10):1587-99.

Jiang H, Wang Y, Viniegra A, Sima C, McCulloch CA, Glogauer M. Adseverin plays a role in osteoclast differentiation and periodontal disease-mediated bone loss. FASEB J. 2015 Jun;29(6):2281-91.

Mohammadi H, Arora PD, Simmons CA, Janmey PA, McCulloch CA. Inelastic behaviour of collagen networks in cell-matrix interactions and mechanosensation. J R Soc Interface. 2015 Jan 6;12(102):20141074.

 

2014

Jane W.C. Cheung, Christopher A.G. McCulloch, J. Paul Santerre. Establishing a gingival fibroblast phenotype in a perfused  degradable polyurethane scaffold: Mediation by TGF-b1, FGF-2, b1-integrin, and focal adhesion kinase. Biomaterials 35 (2014) 10025-10032.

Matthews DC, McNeil K, McCulloch CA, Glogauer M. Adoption issues associated with a new periodontal screening tool: an online  survey of canadian dentists. J Can Dent Assoc. 2014 Aug;80:e57. No PDF available.

Richard D. Speers, Christopher A. McCulloch. Optimizing Patient Safety: Can We Learn From the Airline Industry? J Can Dent Assoc 2014;80:e37. No PDF available.

Bozavikov P, Rajshankar D, Lee W, McCulloch CA. Particle Size Influences Fibronectin Internalization and Degradation by Fibroblasts. Exp Cell Res. 2014 Oct 15;328(1):172-85.

Talior-Volodarsky I, Arora PD, Wang Y, Zeltz C, Connelly KA, Gullberg D, McCulloch CA. Glycated collagen induces a11 integrin expression through TGF-ß2 and Smad3. J Cell Physiol. 2015 Feb;230(2):327-36.

Nogueira-Filho G1, Pesun I, Ploegman C, Wijegunasinghe M, McCulloch CA. Longitudinal Comparison of Cytokines in Peri-Implant Fluid and Gingival Crevicular Fluid in Healthy Mouths. J Periodontol. 2014 Nov;85(11):1582-8.

Wang Q, Wang Y, Fritz D, Rajshankar D, Downey GP, McCulloch CA. Interactions of the protein-tyrosine phosphatase-a with the FAT domain of focal adhesion kinase are involved in IL-1 signaling in fibroblasts. J Biol Chem. 2014 Jun 27;289(26):18427-18441.

Sandkuijl D, Kontenis L, Coelho NM, McCulloch C, Barzda V. Interferometric backward third harmonic generation microscopy for axial imaging with accuracy beyond the diffraction limit. PLoS One. 2014 Apr 7;9(4):e94458

Persadmehr A, Torneck CD, Cvitkovitch DG, Pinto V, Talior I, Kazembe M, Shrestha S, McCulloch CA, Kishen A. Bioactive chitosan nanoparticles and photodynamic therapy inhibit collagen degradation in vitro. J Endod. 2014 May;40(5):703-9.

Aschner Y, Khalifah AP, Briones N, Yamashita C, Dolgonos L, Young SK, Campbell MN, Riches DW, Redente EF, Janssen WJ, Henson PM, Sap J, Vacaresse N, Kapus A, McCulloch CA, Zemans RL, Downey GP. Protein Tyrosine Phosphatase a Mediates Profibrotic Signaling in Lung Fibroblasts through TGF-ß Responsiveness. Am J Pathol. 2014 May;184(5):1489-502.

Hamid Mohammadi and Christopher A McCulloch. Impact of elastic and inelastic substrate behaviors on mechanosensation. Soft Matter, 2014, 10, 408-420.

Mohammadi H, Janmey PA, McCulloch CA. Lateral boundary mechanosensing by adherent cells in a collagen gel system. Biomaterials. 2014 Jan;35(4):1138-49.

Pinto VI, Senini VW, Wang Y, Kazembe MP, McCulloch CA. Filamin A protects cells against force-induced apoptosis by stabilizing talin- and vinculin-containing cell adhesions. FASEB J. 2014 Jan;28(1):453-63.

Goicoechea SM, García-Mata R, Staub J, Valdivia A, Sharek L, McCulloch CG, Hwang RF, Urrutia R, Yeh JJ, Kim HJ, Otey CA.  Palladin promotes invasion of pancreatic cancer cells by enhancing invadopodia formation in cancer-associated fibroblasts. Oncogene (2014) 33, 1265–1273.

2013

Staudinger LA, Spano SJ, Lee W, Coelho N, Rajshankar D, Bendeck MP, Moriarty T, McCulloch CA. Interactions between the  discoidin domain receptor 1 and ß1 integrin regulate attachment to collagen. Biol Open. 2013 Sep 13;2(11):1148-59.

Janmey PA, Wells RG, Assoian RK, McCulloch CA. From tissue mechanics to transcription factors. Differentiation. 2013 Oct;86(3):112-20.

Dhaarmini Rajshankar, Corneliu Sima, Qin Wang, Stephanie R. Goldberg, Mwayi Kazembe, Yongqiang Wang, Michael Glogauer,  Gregory P. Downey, Christopher A. McCulloch. Role of PTPa in the Destruction of Periodontal Connective Tissues. PLoS ONE,  Aug 2013, vol. 8, issue 8, e70659.

Wang Y, Wang Q, D Arora P, Rajshankar D, McCulloch CA. Cell adhesion proteins: roles in periodontal physiology and discovery by proteomics. Periodontol 2000. 2013 Oct;63(1):48-58.

Bartold PM, McCulloch CA. Information generation and processing systems that regulate periodontal structure and function. Periodontol 2000. 2013 Oct;63(1):7-13.

Hossain GS, Lynn EG, Maclean KN, Zhou J, Dickhout JG, Lhoták S, Trigatti B, Capone J, Rho J, Tang D, McCulloch CA, Al-Bondokji I, Malloy MJ, Pullinger CR, Kane JP, Li Y, Shiffman D, Austin RC. Deficiency of TDAG51 Protects Against Atherosclerosis by  Modulating Apoptosis, Cholesterol Efflux, and Peroxiredoxin-1 Expression. J Am Heart Assoc. 2013 May 17;2(3):e000134.

Termei R, Laschinger C, Lee W, McCulloch CA. Intercellular interactions between mast cells and fibroblasts promote  pro-inflammatory signaling. Exp Cell Res. 2013 Jul 15;319(12):1839-51.

Arora PD, Wang Y, Bresnick A, Dawson J, Janmey PA, McCulloch CA. Collagen remodeling by phagocytosis is determined by collagen substrate topology and calcium-dependent interactions of gelsolin with nonmuscle myosin IIA in cell adhesions. Mol Biol Cell. 2013 Mar;24(6):734-47.

Wang Q, Siminovitch KA, Downey GP, McCulloch CA. Ras-guanine nucleotide releasing factors 1 and 2 interact with PLC? at focal adhesions to enable IL-1-induced Ca2+ signaling, ERK activation and MMP-3 expression. Biochem J. 2013 Feb 1;449(3):771-82.

Leszczynska K, Namiot D, Byfield FJ, Cruz K, Zendzian-Piotrowska M, Fein DE, Savage PB, Diamond S, McCulloch CA, Janmey PA, Bucki R. Antibacterial activity of the human host defence peptide LL-37 and selected synthetic cationic lipids against bacteria associated with oral and upper respiratory tract infections. J Antimicrob Chemother. 2013 Mar;68(3):610-8.

Schure R, Costa KD, Rezaei R, Lee W, Laschinger C, Tenenbaum HC, McCulloch CA. Impact of matrix metalloproteinases on inhibition of mineralization by fetuin. J Periodontal Res. 2013 Jun;48(3):357-66.

2012

Li GH, Arora PD, Chen Y, McCulloch CA, Liu P. Multifunctional roles of gelsolin in health and diseases. Med Res Rev. 2012  Sep;32(5):999-1025.

Talior-Volodarsky I, Connelly KA, Arora PD, Gullberg D, McCulloch CA. a11 integrin stimulates myofibroblast differentiation in diabetic cardiomyopathy. Cardiovasc Res. 2012 Nov 1;96(2):265-75.

Rajshankar D, Downey GP, McCulloch CA. IL-1ß enhances cell adhesion to degraded fibronectin. FASEB J. 2012 Nov;26(11):4429-44.

Mohammad I, Arora PD, Naghibzadeh Y, Wang Y, Li J, Mascarenhas W, Janmey PA, Dawson JF, McCulloch CA. Flightless I is a focal adhesion-associated actin-capping protein that regulates cell migration. FASEB J. 2012 Aug;26(8):3260-72.

Shifrin Y, Pinto VI, Hassanali A, Arora PD, McCulloch CA. Force-induced apoptosis mediated by the Rac/PAK/p38 signaling pathway is regulated by filamin A. Biochem J. 2012 Jul 1;445(1):57-67.

Rullo J, Becker H, Hyduk SJ, Wong JC, Digby G, Arora PD, Cano AP, Hartwig J, McCulloch CA, Cybulsky MI. Actin polymerization stabilizes a4ß1 integrin anchors that mediate monocyte adhesion. J Cell Biol. 2012 Apr 2;197(1):115-29.

Park JS, Lee W, McCulloch CA. Intercellular transfer of apoptotic signals via electrofusion. Exp Cell Res. 2012 May  1;318(8):896-903.

Oystein F, McCulloch CA. Impact of Anxiety on Pain Perception Associated with Periodontal and Implant Surgery in a Private Practice. J Periodontol. 2012 Sep;83(9):1079-85.

Greenaway JB, Koehler A, McCulloch CA, Petrik J, Brown TJ, Ringuette MJ. The impact of the ovarian microenvironment on the  anti-tumor effect of SPARC on ovarian cancer (1) (1) This article is part of Special Issue entitled Asilomar Chromatin and has undergone the Journal’s usual peer review process. Biochem Cell Biol. 2012 Feb;90(1):96-107.

2011

Yamashita CM, Dolgonos L, Zemans RL, Young SK, Robertson J, Briones N, Suzuki T, Campbell MN, Gauldie J, Radisky DC, Riches  DW, Yu G, Kaminski N, McCulloch CA, Downey GP. Matrix Metalloproteinase 3 Is a Mediator of Pulmonary Fibrosis. Am J Pathol.  2011 Oct;179(4):1733-45.

Zhang J, Zahir N, Jiang Q, Miliotis H, Heyraud S, Meng X, Dong B, Xie G, Qiu F, Hao Z, McCulloch CA, Keystone EC, Peterson AC, Siminovitch KA. The autoimmune disease-associated PTPN22 variant promotes calpain-mediated Lyp/Pep degradation associated with lymphocyte and dendritic cell hyperresponsiveness. Nat Genet. 2011 Aug 14;43(9):902-7.

Arora PD, Wang Y, Janmey PA, Bresnick A, Yin HL, McCulloch CA. Gelsolin and non-muscle myosin IIA interact to mediate calcium-regulated collagen phagocytosis. J Biol Chem. 2011 Sep 30;286(39): 34184-98.

Wang Q, Downey GP, McCulloch CA. Focal adhesions and Ras are functionally and spatially integrated to mediate IL-1 activation of ERK. FASEB J. 2011 Oct;25(10): 3448-64.

Kim H, McCulloch CA. Filamin A mediates interactions between cytoskeletal proteins that control cell adhesion. FEBS Lett. 2011 Jan 3;585(1):18-22.

2010

Kim H, McCulloch CA. Filamin A mediates interactions between cytoskeletal proteins that control cell adhesion. FEBS Lett. 2011 Jan 3;585(1): 18-22.

Chan MW, Hinz B, McCulloch CA. Mechanical induction of gene expression in connective tissue cells. Methods Cell Biol. 2010;98:178-205.

Atai NA, Bansal M, Lo C, Bosman J, Tigchelaar W, Bosch KS, Jonker A, De Witt Hamer PC, Troost D, McCulloch CA, Everts V, Van Noorden CJ, Sodek J. Osteopontin is up-regulated and associated with neutrophil and macrophage infiltration in glioblastoma. Immunology. 2011 Jan;132(1):39-48.

Wang Q, Rajshankar D, Laschinger C, Talior-Volodarsky I, Wang Y, Downey GP, McCulloch CA. Importance of protein tyrosine phosphatase-{alpha} catalytic domains for interactions with SHP-2 and interleukin-1-induced matrix metalloproteinase-3 expression. J Biol Chem. 2010 Jul 16;285(29):22308-17.

Castella LF, Gabbiani G, McCulloch CA, Hinz B. Regulation of myofibroblast activities: Calcium pulls some strings behind the scene. Exp Cell Res. 2010 Sep 10;316(15):2390-2401.

Goicoechea SM, Bednarski B, Stack C, Cowan DW, Volmar K, Thorne L, Cukierman E, Rustgi AK, Brentnall T, Hwang RF, McCulloch CA, Yeh JJ, Bentrem DJ, Hochwald SN, Hingorani SR, Kim HJ, Otey CA. Isoform-specific upregulation of palladin in human and murine pancreas tumors. PLoS One. 2010 Apr 26;5(4):e10347.

Yuen A, Laschinger C, Talior I, Lee W, Chan M, Birek J, Young EW, Sivagurunathan K, Won E, Simmons CA, McCulloch CA. Methylglyoxal-modified collagen promotes myofibroblast differentiation. Matrix Biol. 2010 Jul;29(6):537-48.

Kim H; Nakamura F, Lee W, Hong C, Pérez-Sala D, McCulloch CA. Regulation of cell adhesion to collagen via ß1 integrins is dependent on interactions of filamin A with vimentin and protein kinase C epsilon. Exp Cell Res. 2010 Jul 1;316(11):1829-1844.

Chan MW, Chaudary FF, Lee W, Copeland JW, McCulloch CA. Force-induced myofibroblast differentiation through collagen receptors is dependent on mammalian diaphanous (mDia). J Biol Chem. 2010 Mar 19;285(12):9273-81.

Kim H, Nakamura F, Lee W, Shifrin Y, Arora PD, McCulloch CA. Filamin A is required for vimentin-mediated cell adhesion and spreading. Am J Physiol Cell Physiol. 2010 Feb;298(2):C221-36.